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Technical Notes
Shorter notes on technical subjects

TN-2016-03: Integrated Michaelis-Menten equation in DynaFit. 3. Application to HIV protease
Determine substrate kinetic constants for the HIV protease by a global fit of combined enzymatic progress curves.
TN-2016-02: Integrated Michaelis-Menten equation in DynaFit. 2. Application to EGFR protein kinase
Determine substrate kinetic constants for 5alpha-ketosteroid reductase from a single enzymatic progress curve.
TN-2016-01: Integrated Michaelis-Menten equation in DynaFit. 1. Application to EGFR protein kinase
A step-by-step instructions and associated data file: Determine substrate kinetic constants for a series of EGFR substrates.
TN-2015-06: Time-dependent inhibition of 5alpha-steroid reductase
PDF (28 pages). A step-by-step DynaFit tutorial: Determination of "slow, tight" enzyme inhibition mechanism and the best-fit values of rate and equilibrium constants..
TN-2015-05: Determination of substrate kinetic parameters from progress curve data
PDF (23 pages). A step-by-step DynaFit tutorial: Determination of Km and kcat from a single well chosen enzymatic progress curve.
TN-2015-04: An algebraic model for the kinetics of covalent enzyme inhibition at low substrate concentrations
PDF (29 pages). A preprint (Analytical Biochemistry) describing a novel mathematical model for the kinetics of covalent enzyme inhibition.
TN-2015-03: King-Altman method in DynaFit
PDF (7 pages). Illustrates the use of the King-Altman method in the DynaFit software package. The software now derives no only rate constants but also "kinetic constants" such as Km and kcat.
TN-2015-02: Automated determination of kinact and Ki for covalent inhibition using the Omnia assay
PDF (117 pages). Accompaniment to a published report [Schwartz et al. (2014) Proc. Natl. Acad. Sci. USA 111, 173178] on covalent inhibition the EGFR kinase.
TN-2015-01: History, variants and usage of the "Morrison equation" in enzyme inhibition kinetics
PDF (18 pages). A brief summary of various historical variants of the "Morrison equation" for initial rates of enzyme reactions under the conditions of tight-binding inhibition.

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Tue Nov 29 06:58:35 2016