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TR2010-01:
A "hit-and-run" kinetic mechanism for the analysis enzyme progress
curves under low substrate concentrations
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Abstract: For enzymatic progress curves conforming to the
Michaelis-Menten mechanism under the experimental
conditions where the substrate concentration is at
least several times smaller than the Michaelis constant,
the minimal fitting model cast as a system of numerically
integrated differential equations is the simple
"hit-and-run" mechanism. The best-fit value of single
relevant rate constant is identical to the specificity constant, kcat/KM.
An illustrative example involves a fluorogenic
continuous assay of the matrix metalloprotease MMP12,
analyzed by the differential-equation oriented software package DYNAFIT.
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Thu May 16 16:21:32 2024 |