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Technical Reports
Longer or highly focussed research reports

TR2010-01: A "hit-and-run" kinetic mechanism for the analysis enzyme progress curves under low substrate concentrations
Abstract: For enzymatic progress curves conforming to the Michaelis-Menten mechanism under the experimental conditions where the substrate concentration is at least several times smaller than the Michaelis constant, the minimal fitting model cast as a system of numerically integrated differential equations is the simple "hit-and-run" mechanism. The best-fit value of single relevant rate constant is identical to the specificity constant, kcat/KM. An illustrative example involves a fluorogenic continuous assay of the matrix metalloprotease MMP12, analyzed by the differential-equation oriented software package DYNAFIT.

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Sat Apr 20 18:22:31 2019