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Integrated Michaelis-Menten equation in DynaFit. Part 1.
Determination of substrate kinetic parameters of EGFR kinase

Integrated Michaelis-Menten equation in DynaFit. 1. Application to EGFR protein kinase

BioKin Technical Note TN-2016-01

Petr Kuzmic
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This brief Technical Note contains detailed step-by-step instructions for how to determine EGFR protein kinase substrate kinetic parameters using a newly proposed mathematical method (Reytor Gonzalez et al., 2016) accepted for publication in the journal Analytical Biochemistry. All kinetic determinations are fully automatic, using a combination of the software package DynaFit (Kuzmic, 1996) and a Perl language interpreter. Detailed instructions and download links are also provided showing how to equip a MS Windows-based computer for the fully automated kinetic analysis.

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Thu May 16 16:21:32 2024