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Integrated Michaelis-Menten equation in DynaFit. Part 2.
Determination of substrate kinetic parameters of 5alpha-ketosteroid reductase

Integrated Michaelis-Menten equation in DynaFit. 2. Application to 5alpha-ketosteroid reductase

BioKin Technical Note TN-2016-02

Petr Kuzmic
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Abstract
The DynaFit software package (http://www.biokin.com/dynafit/) allows the user to specify the theoretical model for an arbitrary bio/chemical mechanism by using a symbolic rather than mathematical notation. However, for convenience, DynaFit also contains a small number of built-in, predefined fitting models. This Technical Note describes a family of predefined algebraic fitting models that describe the time-course of enzyme reactions conforming to the classic Michaelis-Menten kinetic mechanism. An illustrative example includes previously published data on the substrate kinetics of 5alpha-ketosteroid reductase. It is shown that the least squares fit of a single enzymatic progress curve, observed at an optimally chosen substrate concentration, results in a KM value that is exactly identical to the value obtained by using the much laborious and less precise initial rate method.
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Tue Nov 29 06:58:35 2016