


Integrated MichaelisMenten equation in DynaFit. Part 3. 
Determination of substrate kinetic parameters of HIV protease 
Integrated MichaelisMenten equation in DynaFit. 3. Application to HIV protease
BioKin Technical Note TN201603
Petr Kuzmic
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Abstract
The DynaFit software package (http://www.biokin.com/dynafit/) contains a builtin
(“hardcoded”) implementation of the integrated MichaelisMenten equation formulated in
terms of the Lambert omega function. This builtin regression model can be used conveniently
to determine the substrate kinetic parameters either from a single progress curve or from a collection
of enzymatic progress curves analyzed globally. An illustrative example includes previously
published data on the substrate kinetics of HIV protease. It is shown that even a single reaction
progress curve can be used to determine KM and kcat as long as two conditions are met simultaneously:
(1) the initial substrate concentration must be higher than the KM; and (2) the enzyme
reaction must be allowed to proceed to full completion.


