Purpose
Symbolic Notation
Bibliographic Reference
User Manual

Purpose

The main purpose of the program DynaFit is to perform nonlinear least-squares regression of chemical kinetic, enzyme kinetic, or ligand-receptor binding data. The experimental data can be either initial reaction velocities in dependence on the concentration of varied species (e.g., inhibitor concentration vs. velocity), or the reaction progress curves (e.g., time vs. absorbance).

Symbolic Notation

The main advantage in using the program DynaFit is in the ability to characterize the (bio)chemical reacting system in terms of symbolic, or stoichiometric, equations. For example, the ``slow, tight'' inhibition of a dissociative dimeric enzyme is described by the following text:

Monomer + Monomer <==> Enzyme     :  k1  k2
Enzyme + Inhibitor <==> Complex   :  k3  k4
Enzyme + Substrate <==> ReactiveX :  k5  k6
ReactiveX --> Product + Enzyme    :  k7  k8

Bibliographic Reference

If you publish any results obtained by using DYNAFIT, plase cite the following reference:

Kuzmic, P. (1996) Anal. Biochem. 237, 260-273.
"Program DYNAFIT for the Analysis of Enzyme Kinetic Data: Application to HIV Proteinase"

User Manual

Getting Started [PDF]

Describes the user interface and the required formatting of input data files. A worked example requires a sample data file.

Scripting [PDF]

Describes the syntax and semantics of the DynaFit scripting language.