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Program DynaFit
Analysis of (bio)chemical kinetics and equilibria

Purpose Purpose
Symbolic Notation Symbolic Notation
Bibliographic Reference Bibliographic Reference
User Manual User Manual

PurposeTop of pageNext section

The main purpose of the program DynaFit is to perform nonlinear least-squares regression of chemical kinetic, enzyme kinetic, or ligand-receptor binding data. The experimental data can be either initial reaction velocities in dependence on the concentration of varied species (e.g., inhibitor concentration vs. velocity), or the reaction progress curves (e.g., time vs. absorbance).

Symbolic NotationTop of pageNext section

The main advantage in using the program DynaFit is in the ability to characterize the (bio)chemical reacting system in terms of symbolic, or stoichiometric, equations. For example, the ``slow, tight'' inhibition of a dissociative dimeric enzyme is described by the following text:

Monomer + Monomer <==> Enzyme     :  k1  k2
Enzyme + Inhibitor <==> Complex   :  k3  k4
Enzyme + Substrate <==> ReactiveX :  k5  k6
ReactiveX --> Product + Enzyme    :  k7  k8
The names of chemical species ("Monomer", "Enzyme", etc.) are entirely arbitrary and can be freely chosen by the investigator.

Bibliographic ReferenceTop of pageNext section

If you publish any results obtained by using DYNAFIT, plase cite the following reference:

Kuzmic, P. (1996) Anal. Biochem. 237, 260-273.
"Program DYNAFIT for the Analysis of Enzyme Kinetic Data: Application to HIV Proteinase"

User ManualTop of pageNext section
The DynaFit manual is available in two separate volumes:
Download PDF Getting Started [PDF]
Describes the user interface and the required formatting of input data files. A worked example requires a sample data file.
Download PDF Scripting [PDF]
Describes the syntax and semantics of the DynaFit scripting language.

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Thu May 16 16:21:31 2024